| 產品編號 | bsm-61252R |
| 英文名稱 | CTNNA1 Recombinant Rabbit mAb |
| 中文名稱 | α-連環(huán)蛋白重組兔單抗 |
| 別 名 | 102 kDa cadherin-associated protein; alpha E-catenin; CATNA1; CTN1; Cadherin-associated protein; catenin alpha-1; Catenin alpha 1; CTNNA1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:1000-1:5000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 100 kDa |
| 檢測分子量 | 100 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human alpha Catenin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. SWISS: P35221 Gene ID: 1495 細胞粘附蛋白（Call Adhesion Protein） α-catenin是一種多功能的蛋白質，能鏈接E-\N-\P-鈣粘附分子,具有參與細胞粘附和介導信號轉導的雙重功能，并與腫瘤的發(fā)生發(fā)展及浸潤密切相關。 在正常個體中，βα-catenin和鈣黏蛋白形成復合體，介導同型細胞的粘附，維持細胞的穩(wěn)定；同時，α-catenin作為Wnt/α-catenin信號通路的關鍵成員在介導信號轉導過程中調控細胞的增殖和凋亡。 在惡性腫瘤中，α連環(huán)蛋白的表達呈現明顯的異質性，促使細胞異常增殖，還可使細胞之間的黏附性減 |
| 產品圖片 |
Western blot analysis of HeLa cell lysate. Using CTNNA1 (bsm-61252R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
