| 產(chǎn)品編號 | bsm-61254R |
| 英文名稱 | NLRP3 Recombinant Rabbit mAb |
| 中文名稱 | 細胞凋亡誘導蛋白NALP3重組兔單抗 |
| 別 名 | FCU; MWS; FCAS; Cias1; Mmig1; NLRP3; Pypaf1; AII/AVP; AGTAVPRL; Cryopyrin. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 1F5 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | ICC/IF=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 118 kDa |
| 檢測分子量 | 118 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NLRP3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis. SWISS: Q96P20 Gene ID: 114548 |
| 產(chǎn)品圖片 |
4% Paraformaldehyde-fixed THP-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NLRP3) monoclonal Antibody, unconjugated (bsm-61254R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
