| 產(chǎn)品編號 | bsm-61319R |
| 英文名稱 | phospho-CaMK2 alpha (T286) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化鈣/鈣調(diào)素依賴蛋白激酶2α重組兔單抗 |
| 別 名 | Camk2; Camk2a; Camk2b; CAMKA; CaMKII; CaMKIINalpha. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | 50-60 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CaMKII alpha around the phosphorylation site of T286 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation. SWISS: Q923T9 Gene ID: 12325 CaMK2b也是絲/蘇氨基酸激酶的一種，同屬于Ca++/鈣調(diào)素依賴性蛋白質(zhì)激酶家族成員。鈣／鈣調(diào)素依賴性蛋白激酶Ⅱb（CaMKⅡb）廣泛分布于中樞神經(jīng)系統(tǒng)內(nèi)，參與調(diào)節(jié)神經(jīng)遞質(zhì)的合成釋放、信號轉(zhuǎn)導和突觸可塑性的形成以及磷酸化蛋白 |
| 產(chǎn)品圖片 |
Western blot analysis of Mouse brain treated with Lambda phosphatase cell lysate. Using phospho-CaMK2 alpha (T286) (bsm-61319R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
