| 產(chǎn)品編號(hào) | bsm-62797R |
| 英文名稱 | eIF3A Recombinant Rabbit mAb |
| 中文名稱 | 真核翻譯起始因子3A重組兔單抗 |
| 別 名 | EIF3; eIF3 theta; eIF3a; EIF3S10; P167; p180; p185; TIF32. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 167 kDa |
| 檢測(cè)分子量 | 160 |
| 性 狀 | Liquid |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human eIF3A |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. SWISS: Q14152 Gene ID: 8661 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with eIF3A monoclonal antibody, unconjugated (bsm-62797R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF3A Monoclonal Antibody, Unconjugated(bsm-62797R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF3A Monoclonal Antibody, Unconjugated(bsm-62797R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF3A Monoclonal Antibody, Unconjugated(bsm-62797R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with eIF3A Monoclonal Antibody, Unconjugated(bsm-62797R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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