產(chǎn)品編號(hào) | bs-1219R |
英文名稱(chēng) | GRP78 Rabbit pAb |
中文名稱(chēng) | 葡萄糖調(diào)節(jié)蛋白78/熱休克蛋白70蛋白5抗體 |
別 名 | BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN. |
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Specific References (34) | bs-1219R has been referenced in 34 publications.
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研究領(lǐng)域 | 腫瘤 細(xì)胞生物 |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
交叉反應(yīng) | Human,Rat (predicted: Mouse,Pig) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 78 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞漿 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human GRP78: 251-360/654 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. Function: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Subcellular Location: Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Similarity: Belongs to the heat shock protein 70 family. SWISS: P11021 Gene ID: 3309 Database links: Entrez Gene: 3309 Human Entrez Gene: 14828 Mouse Omim: 138120 Human SwissProt: P11021 Human SwissProt: P20029 Mouse Unigene: 605502 Human Unigene: 743241 Human Unigene: 330160 Mouse Unigene: 470180 Mouse Unigene: 11088 Rat 葡萄糖調(diào)節(jié)蛋白78是熱休克蛋白70家族的成員之一,又稱(chēng):熱休克蛋白70蛋白5,作為一種分子伴侶在蛋白質(zhì)的折疊和轉(zhuǎn)運(yùn)過(guò)程及內(nèi)質(zhì)網(wǎng)應(yīng)激反應(yīng)中發(fā)揮重要作用.它在多種腫瘤組織中呈高表達(dá). |
產(chǎn)品圖片 |
Sample:
Lane 1: Spleen (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: U-87MG (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: MDA-MB-231 (Human) Cell Lysate at
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30mi
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