| 產(chǎn)品編號 | bs-0574R |
| 英文名稱 | Dynamin 2 Rabbit pAb |
| 中文名稱 | 酶動力蛋白2抗體 |
| 別 名 | CMTDI1; CMTDIB; Cytoskeletal protein; DNM 2; DNM2; DYN 2; DYN II; DYN2; Dynamin II; Dynamin2; DynaminII; DYNII; DYN2_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞類型標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Cow) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 98 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Dynamin 2: 61-150/870 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Dynamin 2 is a microtubule-associated force-producing protein involved in building microtubule bundles, and it is able to bind and hydrolyze GTP. It is ubiquitously expressed and is likely to be involved in vesicular trafficking processes, especially endocytosis. Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis. Subcellular Location: Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Microtubule-associated. Also found in the postsynaptic density of neuronal cells. DISEASE: Defects in DNM2 are a cause of centronuclear myopathy autosomal dominant (ADCNM) [MIM:160150]; also known as autosomal dominant myotubular myopathy. Centronuclear myopathies (CNMs) are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. CNMs comprise a wide spectrum of phenotypes, ranging from severe neonatal to mild late-onset familial forms. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. Similarity: Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain. SWISS: P50570 Gene ID: 1785 Database links: Entrez Gene: 1785 Human Entrez Gene: 13430 Mouse Omim: 602378 Human SwissProt: P50570 Human SwissProt: P39054 Mouse Unigene: 211463 Human Unigene: 433257 Mouse Unigene: 11231 Rat 發(fā)動蛋白是一種胞質(zhì)溶膠蛋白, 又稱：酶動力蛋白。 能夠同GTP結(jié)合并將GTP水解。 發(fā)動蛋白2(Dynamin 2)是大的鳥苷三磷酸酶（GTPases）和eNOS存在相同的膜區(qū)室。研究顯示Dynamin 2能與eNOS直接作用增強eNOS的活性。Dynamin 2參與Caveolae 的內(nèi)陷活動、小囊的形成與運輸以及受體介導(dǎo)的胞吞作用。 Dynamin 2能和牛主動脈的內(nèi)皮細(xì)胞溶菌產(chǎn)物的eNOS免疫共沉淀。Ca2+轉(zhuǎn)運體刺激的的內(nèi)皮細(xì)胞增強了發(fā)動蛋白免疫共沉淀作用，說明蛋白間的相互作用能夠被細(xì)胞內(nèi)的ca2+瞬變所觸發(fā)。 研究發(fā)現(xiàn)發(fā)動蛋白2的富脯氨酸結(jié)構(gòu)能和eNOS 還原酶結(jié)構(gòu)的FAD部分相互作用，通過促進電子在eNOS還原酶結(jié)構(gòu)的FAD和FMN間相互轉(zhuǎn)移，正性調(diào)節(jié)eNOS的活性。 近期又有研究顯示Dynamin 2能和Caveolin-1發(fā)生直接作用. |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNM2) Polyclonal Antibody, Unconjugated (bs-0574R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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