| 產(chǎn)品編號 | bs-1694R |
| 英文名稱 | DcR2 Rabbit pAb |
| 中文名稱 | 誘捕受體2抗體 |
| 別 名 | TNFRSF10D; Decoy receptor 2; CD 264; CD264; CD-264; DcR 2; decoy with truncated death domain; ID; TNF receptor related receptor for TRAIL; TNF related apoptosis inducing ligand receptor 4; TNFRSF 10D; TRAIL R4; TRAIL receptor 4; TRAIL receptor with a truncated death domain; TRAILR4; TRIALR 4; TRUNDD; Tumor necrosis factor receptor superfamily member 10d decoy with truncated death domain; Tumor necrosis factor receptor superfamily member 10D precursor; TR10D_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 細(xì)胞凋亡 細(xì)胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DcR2: 56-160/386 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L, a member of the TNF family, induces apoptosis of a variety of tumor cell lines. DR4 and DR5 are functional receptors for TRAIL, and DcR1/TRID is a decoy receptor. Another member of the TRAIL receptor family was identified and designated DcR2. The DcR2 receptor is 386 amino acids in length and has an extracellular TRAIL binding domain, but lacks intracellular death domain and does not induce apoptosis. Although this receptor binds to the cytotoxic ligand TRAIL, it contains a truncated death domain and functions as an inhibitory receptor. When overexpressed, the DcR2 receptor can protect cells against TRAIL mediated cytotoxicity. Like DR4 and DR5, DcR2 transcript is widely expressed in a variety of normal human tissues but DcR2 is absent in most tumors. Ultraviolet radiation has been shown to upregulate DcR2 expression on human keratinocytes. Over expression of DcR2 attenuated TRAIL induced apoptosis. Function: Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF-kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway. Subcellular Location: Membrane; Single-pass type I membrane rotein. Tissue Specificity: Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. Similarity: Contains 1 death domain. Contains 3 TNFR-Cys repeats. SWISS: Q9UBN6 Gene ID: 8793 Database links: Entrez Gene: 8793 Human Omim: 603614 Human SwissProt: Q9UBN6 Human Unigene: 213467 Human DcR1因缺乏細(xì)胞內(nèi)信號傳導(dǎo)系統(tǒng)而不能傳遞凋亡信號,但能競爭性與TRAIL（腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體）結(jié)合,有干擾凋亡信號的傳導(dǎo)作用。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human MKN45 cell lysates
Lane 2: Human SW480 cell lysates
Lane 3: Human HUVEC cell lysates
Lane 4: Human DU145 cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-DcR2 (bs-1694R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
Blank control: Jurkat cells(blue). Primary Antibody:Rabbit Anti-DcR2 antibody(bs-1694R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-1694R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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