| 產(chǎn)品編號 | bs-2016R |
| 英文名稱 | Integrin Alpha V + Beta 1 Rabbit pAb |
| 中文名稱 | 整合素αVβ1抗體 |
| 別 名 | Integrin alpha 5 beta 1; fibronectin receptor alpha; ITGAV; CD51; DKFZp686A08142; MSK8; Msk 8; VNR; similar to Integrin alpha-V precursor; Vitronectin receptor subunit alpha; CD51 antigen; HGNC; Vitronectin receptor alpha polypeptide; Vitronectin receptor alpha polypeptide antigen CD51; VNRA; Integrin alpha 5 beta 1; VLA-5; Integrin alpha-5; ITGA5; ITA5_HUMAN; Integrin beta-1; ITGB1; ITB1_HUMAN; |
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Specific References (7) | bs-2016R has been referenced in 7 publications.
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| 研究領域 | 細胞生物 信號轉(zhuǎn)導 細胞膜受體 細胞粘附分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1μg /test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 114 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic Mixed peptides 915-948/1048(human Integrin αV)+ 21-70/798(human Integrin β1) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. Integrin alpha 5 belongs to the integrin alpha chain family. It contains seven FG-GAP repeats. Integrin beta 1 belongs to the integrin beta chain family. It contains one VWFA domain. Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein se ems to enhance angiogenesis in Kaposi's sarcoma lesions. Function: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Subcellular Location: Membrane. Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. SWISS: P06756 Gene ID: 3685 Database links: Entrez Gene: 3685 Human SwissProt: P06756 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: A549 (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Lane 3: Huvec (Human) Cell Lysate at 30 ug
Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Lane 6: MCF-7 (Human) Cell Lysate at 30 ug
Lane 7: Jurkat (Human) Cell Lysate at 30 ug
Primary: Anti-Integrin Alpha V + Beta 1 (bs-2016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 125 kD
Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-Integrin Alpha V + Beta 1 antibody (bs-2016R), Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
