| 產(chǎn)品編號 | bs-6848R |
| 英文名稱 | FSCN1 Rabbit pAb |
| 中文名稱 | 肌動蛋白結(jié)合蛋白Fascin抗體 |
| 別 名 | 55 kDa actin bundling protein; 55 kDa actin-bundling protein; Actin bundling protein; FAN 1; Fscin1; FAN1; Fascin 1; Fascin; Fascin homolog 1 actin bundling protein(Strongylocentrotus purpuratus); Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1_HUMAN; |
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Specific References (1) | bs-6848R has been referenced in 1 publications.
[IF=3.91] Dong, Wei-tao, et al. "iTRAQ proteomic analysis of the interactions between Bombyx mori nuclear polyhedrosis virus and silkworm." Journal of Proteomics (2017). WB ; Other Species.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FSCN1: 401-493/493 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells. Function: Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Subunit: Associates with beta-catenin. Interacts with PLXNB3. Subcellular Location: Cytoplasm, cytoskeleton. Cell projection, filopodium. Cell projection, invadopodium. Cytoplasm, cytosol. Note=In glioma cells, partially colocalizes with F-actin stress fibers in the cytosol. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin. Similarity: Belongs to the fascin family. SWISS: Q16658 Gene ID: 6624 Database links: Entrez Gene: 6624 Human Entrez Gene: 14086 Mouse Omim: 602689 Human SwissProt: Q16658 Human SwissProt: Q61553 Mouse Unigene: 118400 Human Unigene: 289707 Mouse Unigene: 199526 Rat Fascin基因?qū)儆趂ascins家族,其蛋白質(zhì)編碼產(chǎn)物是一種結(jié)構(gòu)獨特、進化保守的肌動蛋白(actin)交聯(lián)蛋白,位于細胞膜皺褶、微棘及應(yīng)力纖維,在各種轉(zhuǎn)化細胞中促使細胞膜突起并增加細胞運動性。近年來研究發(fā)現(xiàn),fascin在許多上皮來源的腫瘤組織細胞中表達上調(diào),在腫瘤的進展中起重要作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fascin) Polyclonal Antibody, Unconjugated (bs-6848R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
