| 產(chǎn)品編號(hào) | bs-12399R |
| 英文名稱 | Multicilin Rabbit pAb |
| 中文名稱 | 多纖毛素蛋白抗體 |
| 別 名 | IDAS; Mci; MCI_HUMAN; Multicilin; Protein Idas. |
| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 干細(xì)胞 細(xì)胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)胞分化 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Multicilin: 281-385/385 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transcription regulator required for multiciliate cell differentiation. Acts by promoting transcription of genes required for multiciliate cell formation. Probably acts in a multiprotein complex (By similarity). Plays a role in mitotic cell cycle progression by promoting cell cycle exit.
Function: Multiciliate cells function prominently in the respiratory system, brain ependyma and female reproductive tract to produce vigorous fluid flow along epithelial surfaces. These specialized cells form during development when epithelial progenitors undergo an unusual form of ciliogenesis, in which they assemble and project hundreds of motile cilia. Notch inhibits multiciliate cell formation in diverse epithelia, but how progenitors overcome lateral inhibition and initiate multiciliate cell differentiation is unknown. Here we identify a coiled-coil protein, termed multicilin, which is regulated by Notch and highly expressed in developing epithelia where multiciliate cells form. Inhibiting multicilin function specifically blocks multiciliate cell formation in Xenopus skin and kidney, whereas ectopic expression induces the differentiation of multiciliate cells in ectopic locations. Multicilin localizes to the nucleus, where it directly activates the expression of genes required for multiciliate cell formation, including foxj1 and genes mediating centriole assembly. Multicilin is also necessary and sufficient to promote multiciliate cell differentiation in mouse airway epithelial cultures. These findings indicate that multicilin initiates multiciliate cell differentiation in diverse tissues, by coordinately promoting the transcriptional changes required for motile ciliogenesis and centriole assembly. Subunit: Homodimer. Interacts with GMNN; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Subcellular Location: Nucleus. Excluded from the nucleolus. DISEASE: Probable target of the anaphase promoting complex/cyclosome (APC/C) which regulates its level in the cell during the mitotic cell cycle. Highly expressed during interphase and early mitosis. Expression decreases during anaphase to become undetectable during telophase and cytokinesis. Similarity: Belongs to the geminin family. SWISS: D6RGH6 Gene ID: 345643 Database links: Entrez Gene: 345643 Human Omim: 614086 Human SwissProt: D6RGH6 Human Unigene: 394578 Human 多纖毛素(multicilin)可以促進(jìn)細(xì)胞長(zhǎng)出許多纖毛。當(dāng)細(xì)胞暴露在多纖毛素中，它們長(zhǎng)出許多纖毛的遺傳機(jī)制被激活。在發(fā)育中的胚胎里，這種蛋白指導(dǎo)某種位于肺部、腎臟和皮膚表面的干細(xì)胞發(fā)育為多纖毛細(xì)胞。如慢性哮喘、肺氣腫和囊腫性纖維化(cystic fibrosis)之類呼吸道疾病的病人經(jīng)常遭受肺部感染，這可能是由于將保護(hù)性粘液從氣管中移走的多纖毛細(xì)胞受損導(dǎo)致的。在未來，干細(xì)胞治療可能用新的纖毛細(xì)胞替換這些受損的細(xì)胞，但是科學(xué)家首先需要知道如何指導(dǎo)干細(xì)胞沿著發(fā)育途徑長(zhǎng)成多纖毛細(xì)胞。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Multicilin Polyclonal Antibody, Unconjugated (bs-12399R) at 1:400 overnight at 4°C, DAB staining.
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