| 產(chǎn)品編號 | bs-10227R |
| 英文名稱 | Nanos3 Rabbit pAb |
| 中文名稱 | 骨巢蛋白抗體 |
| 別 名 | NANOS3; NANOS 3; NANOS-3; NOS3; NOS-3; Nanos homolog 3; NANO3_HUMAN. |
 |
Specific References (1) | bs-10227R has been referenced in 1 publications.
[IF=2.63] Wang Chun. et al. Integrating Network Pharmacology and In Vivo Model to Investigate the Mechanism of Biheimaer in the Treatment of Functional Dyspepsia. EVID-BASED COMPL ALT. 2022;2022:8773527 WB ; Mouse.
|
| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 細胞粘附分子 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Pig,Sheep,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 19 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 細胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nanos3: 101-173/173 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes. Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane. In mammals, two Nidogen proteins, Nidogen and Nidogen-2, interact at comparable levels with Collagen I, IV and Perlecan, serving to stabilize basement membranes and playing a major role in embryogenesis. The two isoforms have a similar shape, consisting of three globular domains, and co-localize in vessel walls and other basement membrane zones. Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, and is widely distributed in basement membranes in heart, placenta, bone and, to a lesser extent, in pancreas, kidney and skeletal muscle. Function: Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions. Subunit: Interacts with LAMA2. Interacts with COL13A1. Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane. Tissue Specificity: Heart, placenta and bone. Less in pancreas, kidney and skeletal muscle. Similarity: Contains 5 EGF-like domains. Contains 5 LDL-receptor class B repeats. Contains 1 NIDO domain. Contains 1 nidogen G2 beta-barrel domain. Contains 2 thyroglobulin type-1 domains. SWISS: Q495E5 Gene ID: 342977 Database links: Entrez Gene: 342977 Human Entrez Gene: 244551 Mouse Omim: 608229 Human SwissProt: P60323 Human SwissProt: Q495E5 Human SwissProt: P60324 Mouse Unigene: 127982 Human Unigene: 216740 Mouse |
| 產(chǎn)品圖片 |
Protein: heart(mouse) lysate at 40ug;
Primary: rabbit Anti-Nanos3 (bs-10227R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 19 kD
Observed band size: 17 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
