產(chǎn)品編號(hào) | bs-14057R |
英文名稱(chēng) | CRIP2 Rabbit pAb |
中文名稱(chēng) | 富含半胱氨酸CRIP2蛋白抗體 |
別 名 | CRIP 2; CRIP; CRIP2; CRIP2_HUMAN; CRP 2; CRP; CRP-2; CRP2; Cysteine rich intestinal protein; Cysteine rich protein 2; Cysteine-rich protein 2; ESP 1; ESP1; ESP1 protein; Hlp; Protein ESP1. |
研究領(lǐng)域 | 心血管 發(fā)育生物學(xué) 轉(zhuǎn)運(yùn)蛋白 結(jié)合蛋白 表觀遺傳學(xué) |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
交叉反應(yīng) | Mouse (predicted: Human,Rat,Horse) |
產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 22 kDa |
檢測(cè)分子量 | |
細(xì)胞定位 | 細(xì)胞核 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CRIP2: 17-120/208 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
This gene encodes a putative transcription factor with two LIM zinc-binding domains. The encoded protein may participate in the differentiation of smooth muscle tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Tissue Specificity: Widespread tissue expression; highest levels in the heart. Similarity: Contains 2 LIM zinc-binding domains. SWISS: P52943 Gene ID: 1397 Database links: Entrez Gene: 1397 Human Entrez Gene: 68337 Mouse Omim: 601183 Human SwissProt: P52943 Human SwissProt: Q9DCT8 Mouse Unigene: 534309 Human Unigene: 70327 Human Unigene: 133825 Mouse Unigene: 4267 Rat |
產(chǎn)品圖片 |
Sample: lung (mouse) Lysate at 40 ug
Primary: Anti-CRIP2(bs-14057R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRIP2) Polyclonal Antibody, Unconjugated (bs-14057R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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