| 產(chǎn)品編號 | bs-16698R |
| 英文名稱 | phospho-IRE1 (Ser724) Rabbit pAb |
| 中文名稱 | 磷酸化IRE1蛋白抗體 |
| 別 名 | IRE1(phospho S724); p-IRE1(phospho S724); Endoplasmic reticulum(ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ER to nucleus signalling |
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Specific References (12) | bs-16698R has been referenced in 12 publications.
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| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 107 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724: RH(p-S)FS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway. Function: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Ubiquitously expressed. High levels observed in pancreatic tissue. Post-translational modifications: Autophosphorylated. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 KEN domain. Contains 1 protein kinase domain. SWISS: O75460 Gene ID: 10595 Database links: Entrez Gene: 10595 Human Entrez Gene: 2081 Human Entrez Gene: 78943 Mouse Omim: 604033 Human SwissProt: O75460 Human SwissProt: Q76MJ5 Human SwissProt: Q9EQY0 Mouse SwissProt: Q9Z2E3 Mouse Unigene: 133982 Human Unigene: 592041 Human Unigene: 700027 Human Unigene: 20452 Mouse Unigene: 340943 Mouse Unigene: 226435 Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Human 293T cell lysates
Lane 2: Human DU145 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human Jurkat cell lysates
Primary: Anti-phospho-IRE1 (Ser724) (bs-16698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 130 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
