| 產(chǎn)品編號 | bs-18156R |
| 英文名稱 | TNNC2 Rabbit pAb |
| 中文名稱 | 肌鈣蛋白C2抗體 |
| 別 名 | Fast skeletal muscle troponin C; skeletal muscle; TNNC 2; TNNC2; TNNC2_HUMAN; Troponin C; Troponin C skeletal muscle; Troponin C type 2(fast); Troponin C type 2; Troponin C2 fast. |
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Specific References (1) | bs-18156R has been referenced in 1 publications.
[IF=4.938] Du J et al.
The regulation of skeletal muscle fiber-type composition by betaine is associated with NFATc1/MyoD.J Mol Med (Berl). 2018 Jun 6. WB ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TNNC2: 2-100/160 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Troponin (Tn), a key protein complex in the regulation of striated muscle contraction, is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase, the Tn-T subunit binds tropomyosin and Tn-C, while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. [provided by RefSeq, Jul 2008] Function: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Similarity: Belongs to the troponin C family. Contains 4 EF-hand domains. SWISS: P02585 Gene ID: 7125 Database links: Entrez Gene: 7125 Human Entrez Gene: 21925 Mouse Omim: 191039 Human SwissProt: P02585 Human SwissProt: P20801 Mouse Unigene: 182421 Human Unigene: 1716 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNNC2) Polyclonal Antibody, Unconjugated (bs-18156R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
