| 產(chǎn)品編號(hào) | bs-18542R |
| 英文名稱 | CCK4/PTK7 Rabbit pAb |
| 中文名稱 | 蛋白酪氨酸激酶7抗體 |
| 別 名 | 8430404F20Rik; CCK 4; CCK-4; Colon carcinoma kinase 4; Human colon carcinoma kinase 4(CCK4) mRNA complete cds; Inactive tyrosine-protein kinase 7; mPTK7/CCK4; OTTHUMP00000217016; Protein tyrosine kinase PTK 7; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK 7; PTK7; PTK7 protein tyrosine kinase 7; PTK7 protein tyrosine kinase; PTK7_HUMAN; Serum response factor; Srf; Tyrosine-protein kinase-like 7. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 激酶和磷酸酶 細(xì)胞粘附分子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 115 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CCK4/PTK7: 371-470/1070 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] Function: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Subcellular Location: Membrane. Cell junction. Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells. Tissue Specificity: Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon. Post-translational modifications: MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway. Similarity: Belongs to the protein kinase superfamily. Cell membrane Tyr protein kinase family. Insulin receptor subfamily.Cell membrane Contains 7 Ig-like C2-type (immunoglobulin-like) domains.Cell membrane Contains 1 protein kinase domain SWISS: Q13308 Gene ID: 5754 Database links: Entrez Gene: 5754 Human Entrez Gene: 71461 Mouse Omim: 601890 Human SwissProt: Q13308 Human SwissProt: Q8BKG3 Mouse Unigene: 90572 Human Unigene: 181833 Mouse Unigene: 198822 Rat |
| 產(chǎn)品圖片 |
Sample:
MCF-7 Cell Lysate at 40 ug
HepG2 Cell Lysate at 40 ug
Primary: Anti- CCK4/PTK7 (bs-18542R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 120 kD
圖片來(lái)自客戶:檢測(cè)到130kDa左右的條帶,Lane1檢測(cè)了293細(xì)胞中的PTK7表達(dá).對(duì)產(chǎn)品評(píng)價(jià)特異性強(qiáng).
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