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CD14 Rabbit pAb (bs-20796R)  
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產(chǎn)品編號(hào) bs-20796R
英文名稱 CD14 Rabbit pAb
中文名稱 內(nèi)毒素受體抗體
別    名 Monocyte Differentiation Antigen 14; CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_HUMAN; Myeloid cell-specific leucine-rich glycoprotein.  
研究領(lǐng)域 轉(zhuǎn)錄調(diào)節(jié)因子  細(xì)胞膜受體  糖尿病  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=3ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 35/40 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD14: 241-300/366 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Aug 2020]

Function:
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Subunit:
Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, MD-2 and TLR4.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Similarity:
Contains 11 LRR (leucine-rich) repeats.

SWISS:
P08571

Gene ID:
929

Database links:

Entrez Gene: 929 Human

Entrez Gene: 12475 Mouse

Omim: 158120 Human

SwissProt: P08571 Human

Unigene: 163867 Human



CD14又稱內(nèi)毒素受體,屬細(xì)胞表面糖蛋白家族成員之一。在機(jī)體免疫、防御系統(tǒng)引起的一系列反應(yīng)中介導(dǎo)細(xì)胞識(shí)別LPS并引起細(xì)胞酪氨酸磷酸化、核因子NF-κB轉(zhuǎn)位、觸發(fā)細(xì)胞因子釋放和氧自由基產(chǎn)生。CD14在細(xì)胞因子產(chǎn)生的調(diào)控方面起重要作用,因而對(duì)于CD14結(jié)構(gòu)、功能及其臨床意義的研究日益受到重視。
產(chǎn)品圖片
Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-CD14 (bs-20796R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 44 kD
Blank control (blue line): HL60 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD14 antibody (bs-20796R),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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