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Mouse Anti-Myc tag  antibody (bsm-33002M)  
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產(chǎn)品編號(hào) bsm-33002M
英文名稱 Mouse Anti-Myc tag  antibody
中文名稱 Myc tag標(biāo)簽單克隆抗體
別    名 avian myelocytomatosis viral oncogene homolog; bHLHe39; c-Myc; class E basic helix-loop-helix protein 39; MRTL; MYC; Myc Epitope Tag; myc proto-oncogene protein; myc-related translation/localization regulatory factor; oncogene c-Myc; proto-oncogene c-Myc; protooncogene homologous to myelocytomatosis virus; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog(avian).  
產(chǎn)品類型 標(biāo)簽抗體 
抗體來(lái)源 Mouse
克隆類型 Monoclonal
克 隆 號(hào) 5E8
交叉反應(yīng) Recombinant protein
產(chǎn)品應(yīng)用 WB=1:2000-10000,ELISA=1:1000-5000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated C-EQKLISEEDL 
亞    型 IgG1
純化方法 affinity purified by Protein G
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Subcellular Location:
Nucleus.

產(chǎn)品圖片
8 ug Tags Positive Control Whole Cell Lysate (bs-41230P) per lane probed with Myc tag monoclonal antibody respectively, unconjugated (bsm-33002M) at 1:5000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
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