| 產品編號 | bsm-54393R |
| 英文名稱 | PICK1 Recombinant Rabbit mAb |
| 中文名稱 | 蛋白激酶Cα相互作用蛋白1重組兔單抗 |
| 別 名 | PICK 1; Pick1; PICK1_HUMAN; PRKCA binding protein; PRKCA-binding protein; Prkcabp; Protein interacting with C kinase 1; Protein interacting with PRKCA; Protein interacting with PRKCA 1; Protein kinase C alpha binding protein; Protein kinase C-alpha-binding protein; Protein that interacts with C kinase 1. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4G1 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PICK1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. Function: Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ACCN3/ACCN2 channel. Subunit: Monomer and homodimer. Subcellular Location: Cytoplasm, perinuclear region. Cell junction, synapse. Note=Also present at excitatory synapses. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylation at Thr-82 appears to inhibit the interaction with AMPA receptors. Similarity: Contains 1 AH domain. Contains 1 PDZ (DHR) domain. SWISS: Q9NRD5 Gene ID: 9463 Database links: Entrez Gene: 9463 Human Entrez Gene: 18693 Mouse Omim: 605926 Human SwissProt: Q9NRD5 Human SwissProt: Q62083 Mouse Unigene: 180871 Human |
| 產品圖片 |
Sample:
Lane 1: SK-Br-3 cell lysate
Lane 2: 293 cell lysate
Primary: Anti-PICK1 (bsm-54393R) at 1:500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution
Predicted band size: 46 kD
Observed band size: 52 kD
Blank control:293T.
Primary Antibody (green line): Rabbit Anti-PICK1 antibody (bms-54393R)
Dilution: 1:100 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1:1000.
Protocol
The ce
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
