| 產(chǎn)品編號 | bsm-51569M |
| 英文名稱 | CREB3L4 Mouse mAb |
| 中文名稱 | CREB3L4單克隆抗體 |
| 別 名 | cAMP responsive element binding protein 3 like 4; JAL; hJAL; ATCE1; CREB3; CREB4; AIBZIP; CR3L4_HUMAN; Cyclic AMP-responsive element-binding protein 3-like protein 4; cAMP-responsive element-binding protein 3-like protein 4; Androgen-induced basic leucine |
| 研究領(lǐng)域 | 染色質(zhì)和核信號 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | F6H12 |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human CREB3L4 between 1-300 amino acids. |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a CREB (cAMP responsive element binding) protein with a transmembrane domain which localizes it to the ER membrane. The encoded protein is a transcriptional activator which contains a dimerization domain, and this protein may function in a number of processing pathways including protein processing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] Function: Transcriptional activator that may play a role in the unfolded protein response. Binds to the UPR element (UPRE) but not to CRE element. Preferentially binds DNA with to the consensus sequence 5'-T[GT]ACGT[GA][GT]-3' and has transcriptional activation activity from UPRE. Binds to NF-kappa-B site and has transcriptional activation activity from NF-kappa-B-containing regulatory elements. Subcellular Location: Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. May also be located in Golgi apparatus. Tissue Specificity: According to PubMed:11830526, exclusively expressed in the prostate. Expressed in breast and prostate cancer cell lines. Expressed in prostatic luminal epithelial cells (at protein level). Expression is significantly more abundant in prostate cancer than in benign prostatic tissue (prostatic hyperplasia). According to PubMed:12111373, also expressed in brain, pancreas and skeletal muscle, and at lower levels in small intestine, testis, leukocyte and thymus. Post-translational modifications: N-glycosylated in the C-terminal region. Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2). PS1 cleavage may be suppressed by a determinant in the C-terminal region. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. Gene ID: 148327 Database links: Entrez Gene: 148327 Human SwissProt: Q8TEY5 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Daudi cell lysates
Lane 2: PC-3 cell lysates
Lane 3: MCF-7 cell lysates
Lane 4: C6 cell lysates
Lane 5: L6 cell lysates
Lane 6: Rat brain tissue lysates
Primary: Anti-CREB3L4 (bsm-51569M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 40 kD
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