| 產(chǎn)品編號(hào) | bsm-60490R |
| 英文名稱 | ACVR1B Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | A8B3 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 跨膜絲氨酸/蘇氨酸激酶激活素1型受體與激活素2型受體形成激活素受體復(fù)合物（ACVR2A或ACVR2B）。將激活素信號(hào)從細(xì)胞表面?zhèn)鬟f到細(xì)胞質(zhì)，從而調(diào)節(jié)許多生理和病理過程，包括神經(jīng)元分化和神經(jīng)元存活、毛囊發(fā)育和循環(huán)、垂體產(chǎn)生FSH、傷口愈合、細(xì)胞外基質(zhì)產(chǎn)生，免疫抑制與癌變。激活素也被認(rèn)為在卵巢卵泡發(fā)育中具有旁分泌或自分泌作用。在受體復(fù)合體中，2型受體（ACVR2A和/或ACVR2B）作為主要的激活素受體，而1型受體（如ACVR1B）作為激活素信號(hào)的下游傳感器。激活素與質(zhì)膜上的2型受體結(jié)合并激活其絲氨酸蘇氨酸激酶。活化的2型受體隨后磷酸化并活化1型受體，例如ACVR1B。一旦激活，1型受體結(jié)合并磷酸化C末端絲氨酸殘基上的SMAD蛋白SMAD2和SMAD3。SMAD2和SMAD3在與激活素受體結(jié)合并隨后磷酸化后不久被釋放到細(xì)胞質(zhì)中，在那里它們與共同的伴侶SMAD4相互作用。這個(gè)SMAD復(fù)合體易位到細(xì)胞核中，在那里它介導(dǎo)激活素誘導(dǎo)的轉(zhuǎn)錄。抑制性SMAD7通過FKBP1A被招募到ACVR1B，可以阻止SMAD2和SMAD3與激活素受體復(fù)合物的結(jié)合，從而阻斷激活素信號(hào)。激活素信號(hào)轉(zhuǎn)導(dǎo)也被通過IGSF1抑制素輔受體與抑制素B受體的結(jié)合所拮抗。ACVR1B也磷酸化TDP2。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 52 kDa
Observed MW: 52 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: U87MG, HepG2
Protein loading quantity: 20 μg
Exposure time: 1 s
Predicted MW: 52 kDa
Observed MW: 52 kDa
Cell line: SH-SY5Y
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60490R
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