| 產(chǎn)品編號 | bsm-60439R |
| 英文名稱 | DYNLL1 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | B3F3 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:5000,IHC-P=1:100-500,IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 作為細(xì)胞質(zhì)動力蛋白1復(fù)合物的幾個非催化輔助成分之一，被認(rèn)為參與連接動力蛋白與貨物和調(diào)節(jié)動力蛋白功能的連接蛋白。細(xì)胞質(zhì)動力蛋白1是微管內(nèi)小泡和細(xì)胞器逆行運動的馬達(dá)，可能在改變或維持細(xì)胞骨架結(jié)構(gòu)的空間分布中起作用。結(jié)合并抑制神經(jīng)元型一氧化氮合酶的催化活性。促進(jìn)ESR1的反式激活功能并在ESR1的核定位中起作用。通過將BCL2L11與微管隔離來調(diào)節(jié)其凋亡活性。在細(xì)胞凋亡的刺激下，BCL2L11-DYNLL1復(fù)合物從細(xì)胞質(zhì)動力蛋白中解離并轉(zhuǎn)移到線粒體，并隔離BCL2，從而中和其抗凋亡活性。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:5000
Primary Ab incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: Neuro-2a, 3：Rat kidney
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 10 kDa
Observed MW: 10 kDa
Tissue: Human colon
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60439R
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