| 產(chǎn)品編號(hào) | bsm-60604R |
| 英文名稱 | Cofilin 1 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | C5E4 |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 與F-肌動(dòng)蛋白結(jié)合并表現(xiàn)出pH敏感的F-肌動(dòng)蛋白解聚活性。與皮質(zhì)下母體復(fù)合體（SCMC）結(jié)合，通過(guò)肌動(dòng)蛋白動(dòng)力學(xué)的調(diào)節(jié)，對(duì)合子在胚胎細(xì)胞分裂過(guò)程中發(fā)揮重要作用。在上皮細(xì)胞的細(xì)胞形態(tài)和細(xì)胞骨架組織的調(diào)節(jié)中起作用。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: MCF-7, 3: 293T, 4: C6,5: NIH/3T3
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 19 kDa
Observed MW: 19 kDa
Cell line: HeLa
Fixation: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary Ab dilution: 1:100
Secondary Ab: Goat Anti-Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60604R
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