| 產(chǎn)品編號(hào) | bsm-60432R |
| 英文名稱 | TSHB Recombinant Rabbit mAb |
| 中文名稱 | 促甲狀腺素β亞單位重組兔單抗 |
| 別 名 | TSHB_HUMAN; Thyrotropin subunit beta; Thyroid-stimulating hormone subunit beta(TSH-B; TSH-beta); Thyrotropin beta chain; Thyrotropin alfa; TSH-β; |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | D7C6 |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-500,Flow-Cyt=1:50-1:100,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Subcellular Location: Secreted. Database links: Entrez Gene: 7252 Human
SwissProt: P01222 Human TSHB（甲狀腺刺激激素亞基Beta）是一種蛋白質(zhì)編碼基因。 與TSHB相關(guān)的疾病包括甲狀腺功能減退，先天性，非甲狀腺功能減退4和中樞性先天性甲狀腺功能減退。其相關(guān)途徑包括通過(guò)GPCR進(jìn)行信號(hào)傳遞和肽激素代謝。與該基因相關(guān)的基因本體論（GO）注釋包括激素活性。該基因的重要旁系同源物是FSHB。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Rat pituitary
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 16 kDa
Observed MW: 16 kDa
Tissue: Rat pituitary
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for bsm-60432R
Cell line: U-2 OS
Fixative: 100% methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60432R
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 90% Methanol
Primary ab dilution: 1:100
Secondary ab: Goat anti Rabbit IgG
Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line).
Isotype control: Rabbit monoclonal IgG (Blue line).
Comment: Line red is the positive signal for bsm-60432R
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