| 產(chǎn)品編號 | bsm-43121M |
| 英文名稱 | MUC16 Mouse mAb |
| 中文名稱 | 卵巢癌抗原單克隆抗體 |
| 別 名 | CA 125; CA-125; CA125 ovarian cancer antigen; Cancer antigen 125; FLJ14303; MUC16_HUMAN; MUC 16; Mucin 16; MUC-16; Mucin 16 cell surface associated; Mucin16; Ovarian cancer related tumor marker CA125; Ovarian carcinoma antigen CA125; Ovarian cancer-related tumor marker CA125 |
| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 淋巴細(xì)胞 b-淋巴細(xì)胞 細(xì)胞骨架 腫瘤細(xì)胞生物標(biāo)志物 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 200 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human MUC16 Protein: 12660-12923/14507 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller “subunits” being described by some investigators. SWISS: Q8WXI7 Gene ID: 94025 Database links: Entrez Gene: 94025 Human SwissProt: Q8WXI7 Human Unigene: 432676 Human
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| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MUC16 monoclonal antibody, unconjugated (bsm-43121M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian mucinous Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated(bsm-43121M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed OVCAR-3 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MUC16) monoclonal Antibody, unconjugated (bsm-43121M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The OVCAR-3 (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-MUC16 antibody (bsm-43121M): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 μg/test. Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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