| 產(chǎn)品編號 | bsm-60646R |
| 英文名稱 | DUSP5 Recombinant Rabbit mAb |
| 中文名稱 | 雙特異性磷酸酶5重組兔單抗 |
| 別 名 | Dual specificity protein phosphatase 5; Dual specificity protein phosphatase hVH 3; Dual specificity protein phosphatase hVH3; DUSP 5; DUSP; HVH 3; HVH3; Serine/threonine specific protein phosphatase; VH 3; VH1 like phosphatase 3; VH3; DUS5_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | R1A10 |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應用 | WB=1:200-1000,IHC-P=1:100-500,IHC-F=1:200-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DUSP5 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, is expressed in a variety of tissues with the highest levels in pancreas and brain, and is localized in the nucleus. [provided by RefSeq, Jul 2008] Subcellular Location: Nuclear. SWISS: Q16690 Gene ID: 1847 Database links:
Entrez Gene: 1847 Human Entrez Gene: 240672 Mouse Omim: 603069 Human SwissProt: Q16690 Human
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| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HCT-116, 2: Raji, 3: K562, 4: C2C12,
5: PC12
Protein loading quantity: 20 ug
Exposure time: 60 s
Predicted MW: 42 kDa
Observed MW: 42 kDa
Tissue: Human papillary thyroid carcinoma
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
bsm-60646R
Tissue: Human bladder carcinoma
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
bsm-60646R
Tissue: Human bladder carcinoma
Section type: Formalin-fixed &
Paraffin-embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
