| 產(chǎn)品編號(hào) | bsm-52441R |
| 英文名稱 | Caspase-1 Recombinant Rabbit mAb |
| 中文名稱 | 天冬氨酸-胱氨酸特異性蛋白酶家族1 重組兔單抗 |
| 別 名 | CASP 1; CASP1; Caspase 1 apoptosis related cysteine peptidase; Caspase 1 apoptosis related cysteine protease; Caspase1; ICE; IL 1 beta converting enzyme; IL 1BC; IL1B convertase; IL1BC; IL1BCE; Interleukin 1 beta convertase; Interleukin 1 beta convertase precursor; Interleukin 1 beta converting enzyme; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; Caspase-1 subunit p20; CASP1_HUMAN. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 5F17 |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant Human Caspase-1 protein: 298-404 /404 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]. Function: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Post-translational modifications: The two subunits are derived from the precursor sequence by an autocatalytic mechanism. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P29466 Gene ID: 834 Database links: Entrez Gene: 834 Human Entrez Gene: 12362 Mouse Omim: 147678 Human SwissProt: P29466 Human SwissProt: P29452 Mouse Unigene: 2490 Human Unigene: 1051 Mouse Unigene: 37508 Rat Caspase-1是半胱氨酸蛋白中的一種酶，參與細(xì)胞凋亡的調(diào)控。它主要用于各種良、惡性腫瘤的研究。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Caspase-1 monoclonal antibody, unconjugated (bsm-52441R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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