| 產(chǎn)品編號 | bs-40295G-IRDye800CW |
| 英文名稱 | Goat Anti-Rabbit IgG H&L, IRDye 800cw conjugated |
| 中文名稱 | IRDye 800cw羊抗兔IgG (H+L)(進口分裝) |
| 別 名 | IRDye? 800CW Goat anti-Rabbit IgG H&L; Immunoglobulin G; 山羊抗兔IgG H&L (IRDye? 800CW)預吸附二抗; ?IRDye 800cw 羊抗兔IgG (H+L); |
| 抗體來源 | Goat |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rabbit |
| 產(chǎn)品應用 | WB=1:10000-30000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1.0 mg/ml |
| 免 疫 原 | Native Rabbit IgG |
| 亞 型 | IgG1 |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
IRDye800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
Producer: Li-Cor(USA)
Product Number: 926-32211
Fluorophore: IRDye 800CW(MW: 1166)
Excitation Wavelength: 778nm(in PBS)
Emission Wacelegth: 795nm(in PBS)
Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgG
IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications. |
| 產(chǎn)品圖片 |
Sample:
Lane 1,4,7: Mouse Testis tissue lysates
Lane 2,5,8: Rat Testis tissue lysates
Lane 3,6,9: Human MCF-7 cell lysates
Primary: Anti-beta-Actin (bs-0061R) at 1/1:2000 dilution
Secondary: IRDye800CW Goat anti-Rabbit IgG (bs-40295G-IRDye8) at 1:50000~200000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
