產(chǎn)品編號 | bsm-41415M |
英文名稱 | Mouse Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid antibody |
中文名稱 | SARS冠狀病毒2核衣殼蛋白單克隆抗體 |
別 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein. |
抗體來源 | Mouse |
克隆類型 | Monoclonal |
克 隆 號 | 14B2D |
交叉反應(yīng) | (predicted: SARS-CoV-2) |
產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 46 kDa |
檢測分子量 | |
性 狀 | Liquid |
濃 度 | ≥1 mg/ml |
免 疫 原 | Recombinant SARS-CoV-2 Nucleocapsid Protei: full length |
亞 型 | IgG2b |
純化方法 | affinity purified by Protein G |
緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
保存條件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
產(chǎn)品圖片 |
Sample:
Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng
Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng
Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng
Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng
Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng
Lane 6:SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng
Primary: Anti- SARS-CoV-2 N protein (bs-41415M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
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