| 產(chǎn)品編號 | bs-0837R-Bio |
| 英文名稱 | Rabbit Anti-Glutathione Reductase/Biotin Conjugated antibody |
| 中文名稱 | 生物素標記的谷胱甘肽還原酶抗體 |
| 別 名 | glutathione reductase; GLUR; Glutathione reductase mitochondrial; GR; Gr1; GRase; GRD 1; GRD1; GSR; MGC78522; GSHR_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 免疫學 激酶和磷酸酶 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glutathione Reductase |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. Function: Maintains high levels of reduced glutathione in the cytosol. Subunit: Homodimer; disulfide-linked. Subcellular Location: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. Database links: Entrez Gene: 2936 Human Entrez Gene: 14782 Mouse Omim: 138300 Human SwissProt: P00390 Human SwissProt: P47791 Mouse Unigene: 271510 Human Unigene: 283573 Mouse Unigene: 19721 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 胱甘肽還原酶為一種黃素酶，可催化氧化型谷胱甘肽轉(zhuǎn)變成還原型谷胱甘肽(GSH)，從而提高細胞液中GSH的含量。 GSH具有抵抗自由基氧化對機體損傷的作用，GSH的重要生理功能在于能還原體內(nèi)生成的過氧化氫(H2O2)。GSH的儲量與創(chuàng)傷、腫瘤等的預后，以及人體衰老有關(guān)。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
