產(chǎn)品編號 | bs-0951R-HRP |
英文名稱 | Rabbit Anti-LH beta/HRP Conjugated antibody |
中文名稱 | 辣根過氧化物酶標記的促黃體生成素抗體 |
別 名 | luteinizing hormone beta; CGB 4; CGB4; hLHB; Interstitial cell stimulating hormone; Interstitial cell stimulating hormone beta chain; Leutropin; LH B; LHB; LSH B; LSH beta; LSHB; LH-B; LSH-B; LSH-beta; Luteinising hormone; Luteinizing hormone beta; Luteinizing hormone beta polypeptide; Luteinizing hormone beta subunit; Luteinizing hormone subunit beta; Lutropin beta chain; Lutropin beta chain precursor; Lutropin subunit beta; LSHB_HUMAN. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領(lǐng)域 | 生長因子和激素 內(nèi)分泌病 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | (predicted: Human, ) |
產(chǎn)品應(yīng)用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 13kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from midlle of human LH beta |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Luteinizing Hormone is a member of the glycoprotein hormone family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. Mutations in the Luteinizing Hormone (LHB) gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. Function: Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. Subunit: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin. Subcellular Location: Secreted. Tissue Specificity: Pituitary gland. DISEASE: Hypogonadism LHB-related (HGON-LHB) [MIM:152780]: Characterized by infertility and pseudohermaphroditism. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the glycoprotein hormones subunit beta family. Database links: Entrez Gene: 3972 Human Omim: 152780 Human SwissProt: P01229 Human Unigene: 154704 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. LH和FSH都是垂體前葉產(chǎn)生的,由兩條多肽鏈(即а和β亞單位)經(jīng)非共價鍵結(jié)合的糖蛋白。LH和FSH都是呈脈沖式分泌,故有較明顯的日內(nèi)和日間變異。 女性月經(jīng)中期的LH高峰促成排卵,男性LH主要刺激Legdig細胞產(chǎn)生睪丸酮,又協(xié)同F(xiàn)SH促進精子成熟。與FSH同時測定,是研究和判斷下丘腦 — 垂體 — 性腺軸功能的常規(guī)檢查方法。LH的測定在預(yù)測排卵時間上具有特殊重要意義,它還可用于絕經(jīng)期檢查,不孕癥診斷和內(nèi)分泌治療的監(jiān)測等。 |
1、抗體溶解方法 | |
2、抗體修復(fù)方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設(shè)計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
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