| 產(chǎn)品編號 | bs-11201R-Bio |
| 英文名稱 | Rabbit Anti-Musashi 1/Biotin Conjugated antibody |
| 中文名稱 | 生物素標(biāo)記的神經(jīng)干細(xì)胞RNA結(jié)合蛋白Musashi1抗體 |
| 別 名 | Musashi 1 / Msi1; Msi 1; Msi1; Msi-1; MSI1H_HUMAN; Musashi homolog 1; Musashi-1; Musashi1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 干細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Rabbit, ) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 39kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Musashi 1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary. Function: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. Similarity: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains. Database links: Entrez Gene: 4440 Human Entrez Gene: 17690 Mouse Omim: 603328 Human SwissProt: O43347 Human SwissProt: Q61474 Mouse Unigene: 158311 Human Unigene: 5077 Mouse Unigene: 162146 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Musashi1是一種進(jìn)化保守的RNA結(jié)合蛋白，在維持干細(xì)胞狀態(tài)、分化和腫瘤發(fā)生方面起著重要作用。Musashi1 選擇性地表達(dá)在神經(jīng)前體細(xì)胞上,包括神經(jīng)干細(xì)胞上。在神經(jīng)系統(tǒng)外，Musashi1還是腸干細(xì)胞的選擇性標(biāo)記。這些組織干細(xì)胞或未成熟細(xì)胞Musashi1的表達(dá)，表明Musashi1在轉(zhuǎn)錄后基因調(diào)節(jié)階段維持這些細(xì)胞未分化狀態(tài)起重要作用。Musashi1過度表達(dá)通過依賴RBP2Jk的旁路激活Notch1，而Notch信號途徑功能為誘導(dǎo)哺乳動物神經(jīng)干細(xì)胞自我更新。通過musashi1-P-小鼠培養(yǎng)腦細(xì)胞的Musashi蛋白產(chǎn)物反義去除研究,發(fā)現(xiàn)這些基因在維持神經(jīng)干細(xì)胞未分化狀態(tài)起著重要的作用。 Musashi抑制m-Numb轉(zhuǎn)錄的分子機(jī)制尚待進(jìn)一步研究。Musashi1有可能除轉(zhuǎn)錄調(diào)控外還參與其他調(diào)控途徑。另外，Musashi1還表達(dá)在一些腦腫瘤的特殊類型(這些腫瘤可能起源自非成熟腦細(xì)胞)，并且表達(dá)水平和腫瘤的惡性程度及增殖能力相關(guān)。 |
