| 產(chǎn)品編號 | bs-3099R-HRP |
| 英文名稱 | Rabbit Anti-Phospho-Cofilin (Ser3)/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標(biāo)記的磷酸化絲切蛋白(Ser3)抗體 |
| 別 名 | Cofilin (Phospho-Ser3); Cofilin (Phospho S3); 18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 生長因子和激素 細胞粘附分子 細胞類型標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, Rat, (predicted: Human, Pig, Cow, Sheep, ) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 18kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]. Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Subunit: Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods. Subcellular Location: Nucleus matrix. Cytoplasm, cytoskeleton. Tissue Specificity: Widely distributed in various tissues. Post-translational modifications: Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Similarity: Belongs to the actin-binding proteins ADF family. Contains 1 ADF-H domain. Database links: Entrez Gene: 1072 Human Entrez Gene: 12631 Mouse Entrez Gene: 494432 Sheep Omim: 601442 Human SwissProt: P23528 Human SwissProt: P18760 Mouse SwissProt: Q6B7M7 Sheep Unigene: 52428 Cow Unigene: 170622 Human Unigene: 329655 Mouse Unigene: 11675 Rat Unigene: 13212 Sheep Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 絲切蛋白(cofilin)是細胞骨架塑形的重要調(diào)節(jié)因素,其主要功能是分解肌動蛋白微絲和增加肌動蛋白單體從肌動蛋白微絲的末端解離的速度,從而促進肌動蛋白微絲的循環(huán),是目前為止已發(fā)現(xiàn)的肌動蛋白結(jié)合蛋白家族中唯一能夠改變肌動蛋白微絲扭曲結(jié)構(gòu)的蛋白. |
