產(chǎn)品編號 | bs-8536R-BF350 |
英文名稱 | Rabbit Anti-Nucleolin/C23/BF350 Conjugated antibody |
中文名稱 | BF350標(biāo)記的核仁蛋白C23抗體 |
別 名 | C23; NCL; Nucl; NUCL_HUMAN; Nucleolin; Protein C23. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領(lǐng)域 | 細(xì)胞生物 染色質(zhì)和核信號 表觀遺傳學(xué) |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human, Rat, (predicted: Mouse, Dog, Pig, Cow, Horse, ) |
產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 76kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Nucleolin |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Nucleolin is the major nucleolar protein of growing eukaryotic cells. C23 (nucleolin, NCL) is a eukaryotic nucleolar phosphoprotein that influences synthesis and maturation of ribosomes. C23 localizes to dense fibrillar regions of the nucleolus. It contains four RNA binding domains that interact with pre-rRNA during synthesis. C23 can influence RNA processing, ribosomal gene transcription and nucleolar targeting of ribosomal components. It is known to associate with a variety of proteins, including the nucleolar protein B23. Phosphorylation by Cdc2 and casein kinase II causes translocation of C23 from the nucleolus to the cytoplasm. Mitotic phosphorylated forms of Bcl-2 are present in nuclear structures in prophase Hela cells together with C23 and Ki-67. Retinoic acid-induced apoptosis leads to C23 down-regulation and Bcl-2 mRNA instability. C23 binds the human telomerase reverse transcriptase subunit (TERT) through interactions with its RNA binding domain 4 and carboxyl-terminal RGG domain, and this interaction is critical for the nucleolar localization of human TERT. Function: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Subunit: Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with APTX and NSUN2. Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains) with TERT; the interaction is important for nucleolar localization of TERT. Interacts with ERBB4. Interacts with GZF1; this interaction is important for nucleolar localization of GZF1. Interacts with NVL. Subcellular Location: Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Post-translational modifications: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). Similarity: Contains 4 RRM (RNA recognition motif) domains. Database links: Entrez Gene: 4691 Human Entrez Gene: 17975 Mouse Omim: 164035 Human SwissProt: P19338 Human SwissProt: P09405 Mouse Unigene: 79110 Human Unigene: 154378 Mouse Unigene: 474153 Mouse Unigene: 144561 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 国产乱伦无码视频 | 精品无码人妻一区二区免费蜜桃 | 亚洲午夜无码久久久久 | 免费看黄色视频麻豆网站 | 97国产精品久久久久久 | 成人AV一二区在线播放 | 色婷婷一区二区三区久久午夜成人 | 蜜桃传媒一级在线播放 | 国产精品扒开腿做爽爽爽视频 | 国产裸体美女免费无遮挡 | 90岁老太婆一级A片 日韩成人AV一区二区 | 一本一道久久综合狠狠躁牛牛影视 | 国产又粗又猛又爽又黄的视频色戒 | 91精品人妻少妇无码影院 | 黄色视频免费的网站 | 成年人黄色视频在线观看 | 刘诗诗毛片一区二区三区 | 色欲狠狠躁天天躁无码中文字幕 | 一本一道久久a久久无码 | 麻花豆传媒剧国产免费mv豆丁网 | 西西www444无码免费视频 | 97人妻人人揉人人澡人 | 中文字幕无码人妻少妇免费 | 中文字幕无码黄色视频 | 亚洲狠狠躁夜夜躁人人爽 | 精品丰满熟女少妇一区二区漫画 | 国产国语对白又又粗又大又爽 | 欧美日韩中文字幕一区二区 | 国产一级A片毛毛天码美女视频 | 国产极品美女无套抽搐高潮91 | 蜜桃AV秘 无码一区二区三区 | 国产寡妇女婬乱毛片视频 | 国产乱妇无码A片免费看视频小说 | 中文字幕永久哔哔免费播放 | 美女扒开尿口让男人桶 | 欧美日韩大陆片一区二区三区 | 中文字幕一本二本迫 | 色五月色婷婷开心五月 | 国产精品无码X88AⅤ | 久久久久久久极品内射 |