產(chǎn)品編號 | bs-17792R-BF647 |
英文名稱 | Rabbit Anti-MPRIP/BF647 Conjugated antibody |
中文名稱 | BF647標記的MPRIP蛋白抗體 |
別 名 | 9530046C02; AA536749; AI647711; C76423; KIAA0864; M-RIP; MGC67316; mKIAA0864; Mprip; MPRIP_HUMAN; Myosin phosphatase Rho-interacting protein; p116 Rho-interacting protein; p116Rip; Rho-interacting protein 3; Rhoip3; RIP3; RP23-180B18.4. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領域 | 細胞生物 免疫學 信號轉(zhuǎn)導 細胞骨架 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | (predicted: Human, Mouse, Rat, Horse, Guinea Pig, ) |
產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 117kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human MPRIP |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: M-RIP is a 1,025 amino acid cytoplasmic and cytoskeletal protein that is required for regulation of the actin cytoskeleton. M-RIP colocalizes with myosin binding subunit (MBS) to regulate the phosphorylation of myosin light chain, and colocalizes with F-actin through its N-terminus in the cytoskeleton. M-RIP also interacts with and RhoA at actin stress fibers via its adjacent coiled coil domains. M-RIP is highly expressed in ovary, with moderate levels found in brain, heart, liver, lung, skeletal muscle, testis and kidney. M-RIP depletion causes an increase of stress fibers in smooth muscle cells, whereas M-RIP over-expression causes disassembly of stress fibers in neuronal cells. Containing two PH domains, M-RIP has multiple phosphorylated serine and threonine residues and exists as three isoforms which are produced by alternative splicing events. Function: Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. Subunit: Binds F-actin through its N-terminus. Interacts with MYZAP. Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains. Subcellular Location: Cytoplasm; cytoskeleton. Colocalizes with F-actin. Similarity: Contains 2 PH domains. Database links: Entrez Gene: 23164 Human Entrez Gene: 26936 Mouse Omim: 612935 Human SwissProt: Q6WCQ1 Human SwissProt: P97434 Mouse Unigene: 462341 Human Unigene: 2402 Mouse Unigene: 2641 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |
| 精品人妻无码一区二区三区蜜桃一 | 无码人妻一区二区三区线花季软件 | 国产裸体美女永久无遮挡 | 亚洲精品白浆高清久久久久久 | 欧美一级搡BBBB搡BBBB | 波多野结衣一区二区视频 | 人妻无码AV天堂二区网站 | 欧美影院内射在线播放 | 人妻体内射精无码视频 | 亚洲BBBB爽爽爽自慰 | 三级片激情免费观看网站 | 少妇人妻系列1~100 | 人人妻人人澡人人爽人 | 一级一A片一a免费看 | 91精品国产黑色丝袜 | 一级毛片在线免费观看 | 日韩无码香港无码台湾无码 | 蜜桃av秘 无码一区二区 | 国产无码一区二区在线观看 | 国产又黄又嫩又滑又白 | AV一区二区在线观看 | 安徽妇搡BBBB搡BBBB袄爱直播 | 国产精品无码ThePorn88 | 91在线无码精品秘 一区 | 久久精品一区二区吉川 | 国产成人精品麻豆传奇 | 特级大胆西西4444人体 | 免费亲子乱婬一级A片 | 久久久 成人网站免费观看 99热成人精品热久久66 | 免费观看一级一片 | 日韩人妻免费视频 | 欧美激情一区二区A片成人牛牛 | 国产一级a毛一级a毛视频在线网站 | 免费无码又爽又色A片 | 久久久亚洲国产视频 | 一级少妇精品内射自慰久久久久 | 性感美女电影院在线观看 | 果冻传媒AⅤ毛片无码蜜桃 91人妻人人澡人人爽人人 | 成人免费视频 国产免费看 ,国产乱人伦无无码视频 | 精国产品一区二区三区A片 91麻豆产精品久久久久久 |