產(chǎn)品編號 | bs-20399R-BF555 |
英文名稱 | Rabbit Anti-HIF-1 Alpha/BF555 Conjugated antibody |
中文名稱 | BF555標(biāo)記的缺氧誘導(dǎo)因子1α /HIF-1α抗體 |
別 名 | ARNT interacting protein; ARNT-interacting protein; Basic helix loop helix PAS protein MOP1; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF 1alpha; HIF-1-alpha; HIF1 A; HIF1 Alpha; HIF1; HIF1-alpha; HIF1A; HIF1A_HUMAN; Hypoxia inducible factor 1 alpha; Hypoxia inducible factor 1 alpha isoform I.3; Hypoxia inducible factor 1 alpha subunit; Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor; Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor); Hypoxia inducible factor1alpha; Hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor-1a; Member of PAS protein 1; Member of PAS superfamily 1; Member of the PAS Superfamily 1; MOP 1; MOP1; PAS domain-containing protein 8; PASD 8; PASD8. |
規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
說 明 書 | 100ul |
研究領(lǐng)域 | 腫瘤 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應(yīng) | Human, Rat, (predicted: Mouse, Chicken, ) |
產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 92kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human HIF-1 Alpha |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. Function: Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP. Subunit: Interacts with COPS5 subunit of COP9 signalosome complex,leading to the regulation of its stability. Interacts with TSGA10(By similarity). Efficient DNA binding requires heterodimerizationof an alpha and a beta/ARNT subunit. Binds to the TAZ-type 1domains of CREBBP and EP300. Interacts with NCOA1, NCOA2, APEX andHSP90. Interacts with VHL which docks HFA1 to the E3 ubiquitinligase complex for subsequent destruction. Interaction, via the ODDdomain, with the beta domain of VHLL, protects HIF1A fromdestruction by competing against the destructive targetinginitiated by VHL. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. Post-translational modifications: In normoxia, is hydroxylated on Pro-402 and Pro-564 in theoxygen-dependent degradation domain (ODD) by EGLN1/PHD1 andEGLN2/PHD2. EGLN3/PHD3 has also been shown to hydroxylate Pro-564.The hydroxylated prolines promote interaction with VHL, initiatingrapid ubiquitination and subsequent proteasomal degradation. Underhypoxia, proline hydroxylation is impaired and ubiquitination isattenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-803 by HIF1AN, thusabrogating interaction with CREBBP and EP300 and preventingtranscriptional activation. S-nitrosylation of Cys-800 may be responsible for increasedrecruitment of p300 coactivator necessary for transcriptionalactivity of HIF-1 complex. Acetylation of Lys-532 by ARD1 increases interaction with VHLand stimulates subsequent proteasomal degradation. Requires phosphorylation for DNA-binding. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains. Database links: Entrez Gene: 3091 Human Entrez Gene: 15251 Mouse Omim: 603348 Human SwissProt: Q16665 Human SwissProt: Q61221 Mouse Unigene: 597216 Human Unigene: 3879 Mouse Unigene: 446610 Mouse
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 缺氧誘導(dǎo)因子1α不僅對于機(jī)體在缺氧條件下維持正常的生理功能具有特別重要的意義,并在腫瘤的生長以及神經(jīng)細(xì)胞凋亡等病理過程中起重要作用. HIF1 alpha能調(diào)節(jié)許多下游基因的表達(dá)水平. 哺乳動物細(xì)胞在低氧壓力條件下出現(xiàn)HIF。HIF是一種轉(zhuǎn)錄因子,對細(xì)胞的缺氧起穩(wěn)定作用。 |
| 男人天堂高清无码 | 安徽妇搡BBBB搡BBBB一 | 操又黄又硬的视频国产 | 97人妻人人爽人人A片 | 老熟女又肥又大黑BBB | 91少妇人妻偷人网站 | 国产农村色情一二三区 | 国产a久久秘 麻豆入口 | Av在线免费网址 | 精品国产乱码久久久久久免费舒淇 | 全免费A级毛片免费看黄瓜视频 | 无码人妻精品一区二区三区蜜臀百度 | 91精品麻豆人妻一区二区 | 草1024榴社区成人影院 | 成人黄色免费在线视频 | 国产一级免费观看 | 精品国产乱码久久久 | 日本三级午夜理伦三级三 | 少妇把腿扒开让我添69式mv | 丰满孕妇高潮一级A片 | 国产一区三区王色视频 | 中国无码丰满人妻啪啪 | 国产成人Av在线播放 | 欧美性受XXXX黑人XYX性爽 | 18 精品 爽爽国产在线观右手 | 色狠狠色综合久久久绯色AⅤ影视 | 亚洲精晶无码成人精品AV | 国产乱子伦一区二区三区 | 无码人妻一区二区三区线花季传件 | 91久久夜色精品国产九色杨思敏 | 午夜精品久久久久久久爽 | 国产精品一区二区三区四区在线观看 | 日本一本二本三区免费 | 国产三级片在线看 | 欧美色图一区二区三区 | 天天日天天射天天干 | 农村妇女一区二区三区视频 | 国产精品高潮呻吟Ⅴ | 亚洲综合激情五月久久 | 中文字幕一区二区三区四区 |